Onboard Analysis of Ballast Water

As contractual agreed, obligatory analytical methods have been further developed for their applicability in the frame of ballast water analysis:

Fluorescence-diacetate Fluorometry – FDA

Chemical and optoelectronical method for the numerical detection of viable plankton organisms in the ballast water, applicable for the IMO size classes >50µm and >10µm<50µm

Pulse Amplitude Modulation Fluorometry – PAM

Optoelectronical method for the determination of the vitality of microalgae via the chlorophyll system of the algal cells, applicable for IMO size classes >50µm and >10µm<50µm, no qualitative (algal species) or quantitative (abundance) data possible, determination is done by ranking of the dimensionless value into a scale of 0,0 to 0,8.

Additionally the BSH agreed that SGS will also further develop these supplementary, analytical methods for ballast water analysis:

Adenosin-triphosphate Fluorometry – ATP

Chemical and optoelectronical method for the numerical detection of viable plankton organisms in the ballast water, applicable for the IMO size classes >50µm and >10µm<50µm, ATP may also be applied to analyze the IMO size class bacteria, however, the results are restricted to quantitative data only.

Fluorescence-in-situ-Hybridisation – FISH

Species specific gene probe hybridization of bacteria followed by qualitative (species) and quantative (abundance) detection of viable bacteria by the aid of a fluorescence microscope, applicable to the IMO size class bacteria only

In addition, for control and validation purposes ballast water samples were analyzed by the optical counting of viable organisms:

Optical counts of viable Plankton organisms

Counts of viable organisms in a defined volume of sample by the aid of specific plankton chambers under the light-microscope, applicable to detect phytoplankton and zooplankton organisms of the IMO size classe >50µm, applicable to detect Zooplankton of the IMO size class >10µm<50µm.

More information

The Facts and Findings of the Project

Onboard Sampling of Ballast Water